| 1. Identificação | |
| Tipo de Referência | Artigo em Revista Científica (Journal Article) |
| Site | mtc-m16c.sid.inpe.br |
| Código do Detentor | isadg {BR SPINPE} ibi 8JMKD3MGPCW/3DT298S |
| Identificador | 8JMKD3MGP8W/34A6RLS |
| Repositório | sid.inpe.br/mtc-m18@80/2008/11.26.13.04 (acesso restrito) |
| Última Atualização | 2009:01.06.19.26.14 (UTC) administrator |
| Repositório de Metadados | sid.inpe.br/mtc-m18@80/2008/11.26.13.04.33 |
| Última Atualização dos Metadados | 2018:06.04.04.05.47 (UTC) administrator |
| Chave Secundária | INPE--PRE/ |
| DOI | 10.1039/b810085c |
| ISSN | 1474-905X |
| Chave de Citação | SchuchGalLimSchMen:2008:DeDNSy |
| Título | Development of a DNA-dosimeter system for monitoring the effects of solar-ultraviolet radiation  |
| Ano | 2008 |
| Mês | November, 20th |
| Data de Acesso | 03 maio 2024 |
| Tipo Secundário | PRE PI |
| Número de Arquivos | 1 |
| Tamanho | 443 KiB |
| 2. Contextualização | |
| Autor | 1 Schuch, André Passaglia 2 Galhardo, Rodrigo da Silva 3 Lima-Bessa, Keronninn Moreno de 4 Schuch, Nelson Jorge 5 Menck, Carlos Frederico Martins |
| Identificador de Curriculo | 1 2 3 4 8JMKD3MGP5W/3C9JHUD |
| Grupo | 1 2 3 4 RSU-CIE-INPE-MCT-BR |
| Afiliação | 1 Department of Microbiology, Isntitute of Biomedical Sciences, University of São Paulo 2 3 4 Instituto Nacional de Pesquisas Espaciais (INPE) |
| Revista | Photochemical & Photobiological Sciences |
| Volume | V. 8 |
| Número | pg. 111 - 120 |
| Páginas | http://www.rsc.org/delivery/_ArticleLinking/ArticleLinking.cfm?JournalCode=PP&Year=2009&ManuscriptID=b810085c&Iss=Advance_Article |
| Histórico (UTC) | 2009-01-07 15:55:02 :: seeger05 -> administrator :: 2010-05-11 01:25:11 :: administrator -> seeger05 :: 2010-05-11 16:15:38 :: seeger05 -> administrator :: 2018-06-04 04:05:47 :: administrator -> seeger05 :: 2008 |
| 3. Conteúdo e estrutura | |
| É a matriz ou uma cópia? | é a matriz |
| Estágio do Conteúdo | concluido |
| Transferível | 1 |
| Tipo do Conteúdo | External Contribution |
| Resumo | Solar radiation sustains and affects all life forms on Earth. In recent years, the increase in environmental levels of solar-UV radiation due to depletion of the stratospheric ozone layer, as a result of anthropogenic emission of destructive chemicals, has highlighted serious issues of social concern. This becomes still more dramatic in tropical and subtropical regions, where the intensity of solar radiation is higher. To better understand the impact of the harmful effects of solar-UV radiation on the DNA molecule, we developed a reliable biological monitoring system based on the exposure of plasmid DNA to artificial UV lamps and sunlight. The determination and quantification of different types of UV photoproducts were performed through the use of specific DNA repair enzymes and antibodies. As expected, a significant number of CPDs and 6-4PPs was observed when the DNA-dosimeter system was exposed to increasing doses of UVB radiation. Moreover, CPDs could also be clearly detected in plasmid DNA when this system was exposed to either UVA or directly to sunlight. Interestingly, although less abundant, 6-4PPs and oxidative DNA damage were also generated after exposure to both UVA and sunlight. These results confirm the genotoxic potential of sunlight, reveal that UVA may also produce CPDs and 6-4PPs directly in naked DNA and demonstrate the applicability of a DNA-dosimeter system for monitoring the biological effects of solar-UV radiation. |
| Área | CEA |
| Arranjo | urlib.net > BDMCI > Fonds > Produção anterior à 2021 > CRCRS > Development of a... |
| Conteúdo da Pasta doc | acessar |
| Conteúdo da Pasta source | não têm arquivos |
| Conteúdo da Pasta agreement | não têm arquivos |
| 4. Condições de acesso e uso | |
| Idioma | en |
| Arquivo Alvo | Development of a DNA-dosimeter system for monitoring ...b810085c André P. Schuch.pdf |
| Grupo de Usuários | administrator seeger05 |
| Visibilidade | shown |
| Detentor da Cópia | SID/SCD |
| Política de Arquivamento | denypublisher denyfinaldraft12 |
| Permissão de Leitura | deny from all and allow from 150.163 |
| 5. Fontes relacionadas | |
| Unidades Imediatamente Superiores | 8JMKD3MGPCW/3EUFCFP |
| URL (dados não confiáveis) | http://xlink.rsc.org/?doi=B810085C |
| Divulgação | WEBSCI; PORTALCAPES. |
| Acervo Hospedeiro | sid.inpe.br/mtc-m18@80/2008/03.17.15.17 |
| 6. Notas | |
| Campos Vazios | alternatejournal archivist callnumber copyright creatorhistory descriptionlevel documentstage e-mailaddress electronicmailaddress format isbn keywords label lineage mark mirrorrepository nextedition notes orcid parameterlist parentrepositories previousedition previouslowerunit progress project readergroup rightsholder schedulinginformation secondarydate secondarymark session shorttitle sponsor subject tertiarymark tertiarytype typeofwork versiontype |
| 7. Controle da descrição | |
| e-Mail (login) | seeger05 |
| atualizar | |